Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis.

Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis.

Fluorescent dye-labeled DNA primers have been developed that exploit fluorescence energy transfer (ET) to optimize the absorption and emission properties of the label. These primers carry a fluorescein derivative at the 5' end as a common donor and other fluorescein and rhodamine derivatives attached to a modified thymidine residue within the primer sequence as acceptors. Adjustment of the dono...

Anchored oligo(dT) primers for automated dye terminator DNA sequencing.

Manual DNA sequencing using fluorescent-labeled primers and a fluorescence scanner.

DNA sequence information is required for many routine applications of current molecular biology. Although sequence data may be generated quickly and efficiently using dedicated automated DNA sequencers, many small laboratories do not have the sequencing demands or finances to justify their purchase. For instance, a primary objective of the U.S. Fish and Wildlife Service’s Fish Genetics Laborato...

Triple fluorescence energy transfer in covalently trichromophore-labeled DNA.

DNA is a unique molecule that can be used to separate donor and acceptor for fluorescence energy transfer (ET)1 and longrange photoinduced electron-transfer studies.2 ET has been used extensively as a spectroscopic ruler for biological structures,3a-c and ET primers and terminators are markedly superior to single dye-labeled reagents in DNA sequencing and analysis.4a-b Molecular beacons using b...

High-throughput AFLP analysis using infrared dye-labeled primers and an automated DNA sequencer.

Amplified fragment length polymorphism (AFLP) analysis is currently the most powerful and efficient technique for the generation of large numbers of anonymous DNA markers in plant and animal genomes. We have developed a protocol for high-throughput AFLP analysis that allows up to 70,000 polymorphic marker genotype determinations per week on a single automated DNA sequencer. This throughput is b...